Table 2 BioSpot counting parameters for the MNA
Parameter | Setting | Parameter | Setting |
|---|---|---|---|
Counting mask size (%) | 85 | Edge Effect Compensation | OFF |
Normalise Counts of Mask | ON | Weight Function Shape | 0.5 |
Smart Count | ON | Edge Compensation Level | 1.0 |
Default sensitivity | 145 | Edge Compensation Before PP | ON |
Min. SpotSize | 0.0051 Sq.mm | Ring-wise Compensation | OFF |
Max SpotSize | 0.2012 Sq.mm | Sensitivity Offset | 50 |
Spot Separation | 10.00 | Border Radius Percentage [%] | 30.0 |
Diffuseness | Largest | Border Outline Percentage [%] | 80.0 |
Overdeveloped area handling | Active | Spot Circularity Filtering | OFF |
Auto Areas | Estimate | Area Weight | 0 |
Manual Areas | Normalized | Circularity Threshold | 1.5 |
Objects | Normal, diffuse spots, high background | Maximum area of filtered spots [pix] | 500.0 |
Background balance | ON (80) | Use Global Object Enhancement | OFF |
Fill Holes | OFF | Method | Enhanced Separation |
‘Fiber’ Removal | ON | Aperture [pix] | 20 |
Spots Removal | Inactive | Weight | 3.0 |
ROI-Touching Removal | OFF | Enhanced Separation Level | 0 |
