Extended Data Fig. 3: RRBS performance metrics and global CpG methylation across 80 passing cells.
From: Smart-RRBS for single-cell methylome and transcriptome analysis
Notched box plots to show the overall and inner-quartile range, median and 95% confidence interval of the number of purity filtered and fully demultiplexed RRBS reads before and after genome alignment (total and unique alignments) (a), the C-to-U bisulfite conversion rate of presumably unmethylated cytosines in non-CpG (CpH) context covered by RRBS reads (b) and the mean methylation level at all CpG sites covered in a given cell (c). d, Venn diagrams showing median and mean numbers of CpG sites covered per passing cell exclusively by reads from the large or small size fraction or covered by both. The approximate total numbers of passing read pairs in 80 passing cells from large and small size fractions were 83 million and 90 million, respectively. Aligned and uniquely aligned read numbers for each size fraction are available in the source data for this figure.
