Extended Data Fig. 4: RNA-seq performance metrics across 80 passing cells.
From: Smart-RRBS for single-cell methylome and transcriptome analysis
Notched box plots to show the overall and inner-quartile range, median and 95% confidence interval of purity-filtered demultiplexed RNA-seq reads before and after alignment to the genome (total and unique alignments) by using STAR55 v.2.7.3a with the arguments --quantMode GeneCounts --sjdbGTFtagExonParentGene gene_name --outFilterScoreMinOverLread 0.1 --outFilterMatchNminOverLread 0.1 (a), the library size of unique reads aligning to exons (b) and the fraction of exonic reads that map to the mitochondrial genome (c).
