Extended Data Fig. 3: Gel electrophoretic analysis and flow cytometric validation of mOrange_GG plasmid.
From: Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters
a, Gel electrophoretic analysis of mOrange_GG plasmid. Lane 1: NEB 1-kb MW ladder; lane 2: PMax_mOrange_G299C ccDNA; lane 3: HIA overnight extension reaction for mOrange_GG; lane 4: mOrange_GG after a 3-h digestion with T5 Exo; lane 5: mOrange_GG after PEG precipitation step; and lane 6: final mOrange_GG ccDNA after T5 Exo and PEG purification steps. b, Flow cytometric quantitation of percent reporter expression and normalized relative reporter expression in TK6 cells compared to MMR-deficient MT1 lymphoblastoid cells. Error bars represent s.e.m. from three to four biological replicates; differences of statistical significance (***, P < 0.005) were determined by unpaired two-tailed t test.
