Extended Data Fig. 2: Promoter region and reporter plasmid used during SorTn-seq. | Nature Protocols

Extended Data Fig. 2: Promoter region and reporter plasmid used during SorTn-seq.

From: SorTn-seq: a high-throughput functional genomics approach to discovering regulators of bacterial gene expression

Extended Data Fig. 2

Type III-A CRISPR-Cas expression was measured from an eYFP fusion to 250 nt upstream of the csm operon. Key features in the promoter are indicated (−35 and −10) as are the transcription start site (+1), native RBS and the start codon (ATG, green). An IPTG-inducible 2nd fluorophore (mCherry) is under the control of the T5-lac promoter (PT5-lac). The pBR322 origin of replication facilitates ~15–20 copies of the reporter per cell.

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