Extended Data Fig. 4: Two-photon six-color spectral imaging.
From: Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D
a, 3D rendering of a human pediatric Wilms tumor sample labeled with DAPI (not shown), KI67 (cyan), NCAM1 (blue), SIX2 (green), CDH1 (red) and F-ACTIN (signal intensity gradient: black-red-orange-yellow). b, Wilms tumor dataset labeled with DAPI (not shown), KI67 (cyan), NCAM1 (blue), SIX2 (green), CDH1 (red) and F-ACTIN (gray). Scale bar, 100 μm. c, Single-channel optical sections of the same Wilms tumor dataset. Scale bars, 100 μm.
