Extended Data Fig. 2: Clearing comparison.
From: Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D
a, Widefield microscopic images of human embryonic kidney tissues uncleared or cleared with FUnGI, Ce3D, CUBIC or iDISCO. Squares represent 1 mm. b, Optical sections at two depths (surface and 100 μm) for all eight channels per condition. Red asterisks indicate lack of specific signal in this channel. Scale bars, 50 μm. c, Quantification of the 10% highest intensity pixels per z-plane per channel for every condition. Channels without sufficient specific signal indicated with a red asterisk were not quantified.
