Extended Data Fig. 2: Steps for preparation of the cell syringe with tubing directly attached to the syringe without a needle. | Nature Protocols

Extended Data Fig. 2: Steps for preparation of the cell syringe with tubing directly attached to the syringe without a needle.

From: A microfluidic Braille valve platform for on-demand production, combinatorial screening and sorting of chemically distinct droplets

Extended Data Fig. 2

a, Preparation based on a needle: a 23 G needle is used and the metal part is heated so that it can be removed from the plastic part. In the top of the plastic part, a hole is punched using a 0.75 mm puncher, then the cell tubing is inserted and fixed by UV glue. Finally, the syringe is autoclaved. b, Alternative method for syringe preparation based on PDMS: PDMS is mixed using elastomer and curing agent in a ratio of 1:10 in a plastic beaker, and syringes are inserted into the PDMS. The PDMS is degassed in a desiccator and cured overnight at 65 °C. Syringes are separated using a scalpel, and the cell tubing is inserted into the PDMS, so that the end of the tubing is outside the PDMS. The tube is fixed with UV glue and autoclaved. c, Setup for stirring cells: ice pack and a magnetic stirrer are placed on top of the cell syringe to ensure cooling and agitation of cells (here we used a VP710 magnetic tumble stirrer as also used in DropSeq1, but more conventional stirrers turned upside down can be used as well).

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