Extended Data Fig. 4: Calculating signal intensity of trachea, heart and air for automatic lung segmentation with Analyze software.
From: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice
a, Calculation of the mean signal intensity of trachea and heart by setting parameters. b–d, Images showing step-by-step instructions to calculate signal intensity of air inside the lung starting with creating a calibration curve with results from the trachea and the heart (b), loading Image algebra from selected lung scan (c) and then dragging image into Input picture (arrow) followed by filling equation from the calibration curve for Output and naming output image with underscore (_) at the end (d). e, Image showing how to segment the lung from the background by setting threshold in region grow.
