Extended Data Fig. 2: Calculating the wound area by using the Fiji (Image J) Software.
From: Reproducible strategy for excisional skin-wound-healing studies in mice
a–c, Open high-resolution scan in Fiji (a) and select the ‘Make Binary’ command to transform the image to white & black (b and c). The red square in c defines the selected zoomed-in area in D. d–g, Zoom-in to detect small gaps in the outlines (the purple-dashed square marks the gapped area). Use the hand-free selection tool (d, blue square) and the ‘Clear’ command to fill the gaps. Use the ‘Wand’ tool (g, blue square) to select the area you wish to calculate and press ‘M’ on your keyboard to perform the measurements. The values will be shown in a pop-up window and can be copied directly to an Excel file.
