Extended Data Fig. 5: Intracellular polymerization inhibited proliferation.
a, Colony growth assay depicting the impact of intracellular polymerization on the proliferative ability of HeLa cells. Left panel shows cells treated with PBS in the absence of light, while the right panel displays cells under similar conditions but with light exposure. The scale bar is indicative of 1 cm. b, Representation of cell cycle phases for HeLa cells subjected to treatments with or without intracellular polymerization. The four groups depicted are cells treated with PBS in the absence and presence of light, and cells treated with PC1 in the absence and presence of light. The percentages of cells in the G0/G1, S, and G2/M phases of the cell cycle are presented. Data were based on n = 6 samples. c, Immunoblotting assays highlight the expression levels of key cell cycle regulators, specifically Cyclin B1 and Cyclin E1, in HeLa cells under different treatment conditions. GAPDH serves as the loading control, and the molecular weight (in kDa) of each protein is denoted on the right. Statistical evaluations were executed using one-way ANOVA accompanied by a Dunnett post-test in comparison with the untreated control groups. Levels of significance are denoted as: *P < 0.05, ***P < 0.001, ****P < 0.0001, ns (not significant). Figure reproduced with permission from ref. 20, American Chemical Society.
