Extended Data Fig. 1: Exemplary production of hiPSC-derived cardiomyocytes from vitronectin and (GMP-conforming) CTS vitronectin pre-culture.
From: Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks
a and b, Appearance of cells on CTS vitronectin (a) and vitronectin (b) on day −2 of the production protocol in monolayer (scale bar, 500 µm). c, Expression of markers of an undifferentiated state at CP 0 before induction of differentiation in samples from spinner flasks pre-cultured on vitronectin or CTS vitronectin. d, Cell yield at CP I of respective spinner-flask runs. e, Aggregate diameter of representative samples taken at CP I from respective spinners. Shown are individual values and mean ± s.d. in red. f, Percentage of cells positive for cardiac markers in representative samples from the respective spinner flasks at CP I.
