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CRISPR–Cas genome editing

Integration of large genetic payloads using prime editing and site-specific integrases

Nature Protocols volume 20, pages 1391–1392 (2025)Cite this article

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A recent protocol for PASTE (programmable addition via site-specific targeting elements) combines prime editing and site-specific integrases to integrate large genetic payloads.

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Fig. 1: Prime editing combined with an integrase mediates targeted insertion of large genetic payloads.

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Authors and Affiliations

  1. GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, Guangzhou Medical University, Guangzhou, People’s Republic of China

    Zongliang Gao

  2. Department of Biomedicine, Aarhus University, Aarhus, Denmark

    Rasmus O. Bak

Authors
  1. Zongliang Gao
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  2. Rasmus O. Bak
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Correspondence to Rasmus O. Bak.

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Competing interests

R.O.B. is a co-inventor of patents related to CRISPR/Cas genome editing, reports research funding from Novo Nordisk, and is a co-founder of, holds equity in, and is a paid consultant to UNIKUM Tx.

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Gao, Z., Bak, R.O. Integration of large genetic payloads using prime editing and site-specific integrases. Nat Protoc 20, 1391–1392 (2025). https://doi.org/10.1038/s41596-024-01094-9

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