CellPhoneDB infers cell–cell communication by integrating single-cell genomics data with a curated database of ligand–receptor interactions. Now, a Protocol Update describes the latest version of the tool including many new features.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$32.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout

References
Armingol, E., Baghdassarian, H. M. & Lewis, N. E. Nat. Rev. Genet. 25, 381–400 (2024).
Troulé, K. et al. Nat. Protoc. https://doi.org/10.1038/s41596-024-01137-1 (2025).
Vento-Tormo, R. et al. Nature 563, 347–353 (2018).
Herholt, A., Sahoo, V. K., Popovic, L., Wehr, M. C. & Rossner, M. J. Curr. Opin. Chem. Biol. 66, 102091 (2022).
Dimitrov, D. et al. Nat. Cell Biol. 26, 1613–1622 (2024).
Jin, S., Plikus, M. V. & Nie, Q. Nat. Protoc. 20, 180–219 (2025).
Browaeys, R., Saelens, W. & Saeys, Y. Nat. Methods 17, 159–162 (2019).
Garcia-Alonso, L. et al. Nat. Genet. 53, 1698–1711 (2021).
Garcia-Alonso, L. et al. Nature 607, 540–547 (2022).
Marečková, M. et al. Nat. Genet. 56, 1925–1937 (2024).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
About this article
Cite this article
Pinheiro-de-Sousa, I., Petsalaki, E. Advancing cell–cell communication analysis from single-cell genomics data. Nat Protoc (2025). https://doi.org/10.1038/s41596-025-01140-0
Published:
DOI: https://doi.org/10.1038/s41596-025-01140-0