CRISPR screens are widely used to identify essential genes and potential drug targets, but typical setups were designed for the study of highly proliferative cancer cell lines. Now, a protocol, using inducible Cas9, enables screens of non-proliferative cell states including senescence, quiescence and terminal differentiation, thereby expanding the applicability of CRISPR screens.
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References
Bock, C. et al. Nat. Rev. Methods Primers 2, 8 (2022).
Colville, A. et al. Cell Metab. 35, 1814–1829.e6 (2023).
Casagrande Raffi, G. et al. Nat. Protoc. https://doi.org/10.1038/s41596-025-01251-8 (2025).
Wang, L. et al. Nat. Cancer 3, 1284–1299 (2022).
Chen, M. et al. Cell Rep. Med. 5, 101471 (2024).
Montero, J. J. et al. Nat. Methods 21, 584–596 (2024).
Esk, C. et al. Science 370, 935–941 (2020).
Braun, C. J. et al. Nat. Protoc. 17, 1903–1925 (2022).
Tian, R. et al. Nat. Neurosci. 24, 1020–1034 (2021).
Johmura, Y. et al. Science 371, 265–270 (2021).
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Bugter, J.M., Rad, R. Investigating non-proliferative cell states with inducible CRISPR screens. Nat Protoc (2025). https://doi.org/10.1038/s41596-025-01252-7
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DOI: https://doi.org/10.1038/s41596-025-01252-7
