The complex spatiotemporal signaling of human neural tube development is successfully engineered in a device using microfluidic gradients, creating a high-fidelity model of the developing human central nervous system.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$32.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Birtele, M., Lancaster, M. & Quadrato, G. Nat. Rev. Mol. Cell Biol. 26, 389–412 (2024).
Xue, X. et al. Nat. Protoc. https://doi.org/10.1038/s41596-025-01266-1 (2025).
Xue, X. et al. Nature 628, 391–399 (2024).
Urenda, J. P., Del Dosso, A., Birtele, M. & Quadrato, G. Biol. Psychiatry 93, 606–615 (2023).
Rifes, P. et al. Nat. Biotechnol. 38, 1265–1273 (2020).
Karzbrun, E. et al. Nature 599, 268–272 (2021).
Bagley, J. A., Reumann, D., Bian, S., Lévi-Strauss, J. & Knoblich, J. A. Nat. Methods 14, 743–751 (2017).
Birey, F. et al. Nature 545, 54–59 (2017).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
About this article
Cite this article
Serles, P., Quadrato, G. Microfluidic gradients create a stem cell model of the human central nervous system. Nat Protoc (2025). https://doi.org/10.1038/s41596-025-01269-y
Published:
DOI: https://doi.org/10.1038/s41596-025-01269-y
