Fig. 3

Quality assessment of the human immune cell library. Retention time (RT) fit of +2 and +3 charge states of the sample peptides in the spectral library (a), Number of observed b- and y-ion fragments in the spectral library (b), distribution of fragment ions by observed fragment charge states in the spectral library (c), distribution of precursors by precursor charge states (d), distribution of precursors by precursor m/z values across the acquired mass range in the assay library (e), distribution of the observed peptides by peptide length in the assay library (f), distribution of precursors by the number of fragment ions per precursor (g), and distribution of proteins by the number of peptides per protein in the spectral library (h).