Table 2 Primers and PCR conditions used to amplify targeted gene regions.

From: Fish databases for improving their conservation in Colombia

Locus

Primers

[MgCl2]

Temperature alignment

Reference

COX1

FISH-COI_F1 TCAACCAACCACAAAGACATTGGCAC

2.5 mM

56 °C

70

FISH-COI_R1 TAGACTTCTGGGTGGCCAAAGAATCA

12S

L941-PHE AAATCAAAGCATAACACTGAAGATG

2.5 mM

48 °C

69

H2010-VAL CCAATTTGCATGGATGTCTTCTCGG

16S

16Sa-L ACGCCTGTTTATCAAAAACAT

2.5 mM

57 °C

71

16Sb-H CCGGTCTGAACTCAGATCACGT

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