Fig. 1
From: Chromosome-level genome assembly and annotation of Japanese anchovy (Engraulis japonicus)
The overview of the chromosome-level genome assembly and annotation. Chrs: chromosomes. We first used 95.0 Gb short-read sequencing data to predict the assembled genome size was approximately 1,045.1 Mb by K-mer analysis, and the repeat sequences and heterozygosity were approximately 54.0% and 2.3%, respectively. Then, the 51.3 Gb of PacBio ccs data resulted in a 1,467.6 Mb assembly, with contig N50 of 456.3 kb. The contigs were anchored into 24 pseudo-chromosomes covering roughly 95.2% of the genome assembly with the assistance of 109.5 Gb Hi-C reads. The final assembly consisted of 24 pseudo-chromosomes that yielded 1,423.3 Mb of E. japonicus genome, with a scaffold N50 of 55.0 Mb. The genome contained 54.9% repeat sequences and 23,709 genes were functionally annotated from a total of 24,405 (97.15%) predicted protein-coding genes by combination of RNAseq and ISO-Seq annotation, genome sequence, and homolog protein.
