Table 2 2D cell culture conditions.
Cell Line | Supplier | 2D Culture Condition | STR profile confirmation (Yes/No) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Medium | Glucose concentration (g/l) | Antibiotics (% P/S) | HEPES (mM) | Other Supplements | Serum Type | Serum concentration (%) | Heat-inactivated (Yes/No) | CO2 (%) | |||
4T1 | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | N/A |
4T1LUC | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | N/A |
A549 | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
ASC | Sigma | DMEM HG, Glutamax | 4.5 | 0.5 | — | — | FBS | 10 | Yes | 5 | Yes |
BEAS2B | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
BT20 | ATCC | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
BT474 | Tumorbank Heidelberg | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
CAL33 | Leibniz Institute DSMZ | DMEM LG | 1 | 1 | 25 | — | FBS | 10 | Yes | 5 | Yes |
CT5.3 hTERT | Primary, immortalized (hTERT transfection) | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 10 | Yes |
D2A1LUCmCherry | Gift from Clare Isacke, London, UK | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | N/A |
DLD1 | ATCC | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
FaDu | ATCC (Dresden subline) | DMEM LG | 1 | 1 | 25 | — | FBS | 10 | Yes | 8 | Yes |
H1299 | ATCC | RPMI | 2 | / | — | 1% Glutamine, 0,1% insulin | FBS | 10 | No | 5 | Yes |
HCA2hTERT | Gift from C. Jones, Cardiff, UK | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
HCC2998 | NCI | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
HCT15 | ATCC | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
HCT116 | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
HEK293 | ATCC | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
HeLa | Tebu-bio | RPMI | 2 | 1 | — | — | FBS | 10 | No | 5 | Yes |
HEPG2 | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
HOP62 | NCI | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
Hs578T | ATCC | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
HSC4 | JCRB/HSRRB (Japan) | DMEM LG | 1 | 1 | 25 | — | FBS | 10 | Yes | 5 | Yes |
HuH7 | CLS (Cell line service GmbH, Eppelheim, Germany) | RPMI | 2 | 1 | — | — | FBS | 10 | No | 5 | Yes |
LNCap | CLS | RPMI | 2 | 1 | — | — | FBS | 10 | No | 5 | Yes |
MCF7 | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
MCF10A | ATCC | DMEM/F12 | 3.15 | 1 | — | 0,5 µg/ml hydrocortisone; 20 ng/ml HB-EGF; 100 ng/ml Cholera Toxin; 10 µg/ml insulin | Horse Serum | 5 | No | 5 | Yes |
MDAMB231 | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
MDAMB361 | ATCC | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
MDCK | CLS | RPMI | 2 | 1 | — | — | FBS | 10 | No | 5 | N/A |
MRC5 | ATCC | EMEM | 1 | / | — | — | FBS | 10 | No | 5 | Yes |
NCIH226 | ATCC | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
NCIH460 | ATCC | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
OVCAR8 | NCI | DMEM LG | 1 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
PANC1 | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
PaTu8902 | DSMZ | DMEM LG | 1 | 1 | 25 | — | FBS | 10 | Yes | 8 | Yes |
SAR030 | Primary, early-passage | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
SAR121 | Primary, early-passage | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
SAS | JCRB/HSRRB (Japan) | DMEM LG | 1 | 1 | 25 | — | FBS | 10 | Yes | 8 | Yes |
SKOV3 | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
SW837 | ATCC | DMEM/F12 | 3.15 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
SW1353 | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
T47D | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
U87MG | ATCC | DMEM HG | 4.5 | 1 | — | — | FBS | 10 | Yes | 5 | Yes |
U138MG | CLS | MEM | 1 | 1 | 25 | — | FBS | 10 | Yes | 5 | Yes |
U251MG | CLS | MEM | 1 | 1 | 25 | — | FBS | 10 | Yes | 5 | Yes |
VCaP | ATCC | DMEM HG | 4.5 | / | — | — | FBS | 10 | No | 5 | Yes |
