Fig. 2
From: Chromosome-level reference genome assembly for the protected resource plant, Zenia insignis
Flowchart of Zenia insignis genome assembly. (A) The pipeline of genome size survey for Zenia insignis. Two methods were used to predict the genome of Zenia insignis: flow cytometry and genome survey. (B) The pipeline of assembly process for three assembly software and two datasets. In this study, three assembly software, Falcon, Canu, and Flye, were utilised to assemble the redundant and non-redundant data, respectively. “Falcon_contig” denotes the result of assembling the redundant dataset using Falcon software, “Falcon_purge_contig” denotes the result of assembling the non-redundant dataset using Falcon software, and so forth. The results obtained from the assembly were evaluated using N50, BUSCO and LAI. (C) The pipeline of Hi-C assembly. Hi-C assembly was first performed on the redundant dataset, and the Falcon_purge_contig dataset was considered the best assembly result through interaction heatmap analysis combined with N50, BUSCO and LAI scoring. Further manual adjustments were made to obtain a high quality genome of Zenia insignis.
