Fig. 3

Analysis of inter-plate variation across mimic and inhibitor screen plates. Cell count (upper panels) and % cells infected (lower panels) are represented as FC to siOTP-NT, across all screen plates (10 mimic and 8 inhibitor). Data is shown for miRNAs in addition to screen controls (uninfected, mock, siOTP-NT, siTOX, and siPLK1) and noting the last mimic plate had a portion of the plate with no targets (defined as empty). Screen outcomes were highly concordant with the exception of the replicates of plate 19055 from the inhibitor library which showed an increase in % cells infected in replicate B. As mentioned in the Technical Validation section, these wells were flagged as discordant and should be treated with caution.