Fig. 5
B. mori cocoon shell lipidomics. (a) A circus plot displaying the proportion of each super-class or sub-class of lipids in relation to the age of the cocoon shell or the presence/absence of colour. (b) Decision tree analysis47 showing the chemical structure of CAR 22:1 and the signal intensity fraction above or below which old or contemporary samples can be distinguished. (c) Validation of the presence of lipids in cocoon shells. A set of 10 contemporary (Black bars) and 10 old (White bars) cocoon shell samples were analysed for the presence of lipids as identified in the lipidomics analysis (see Fig. 5a). Results are expressed as oleic acid equivalents per cocoon shell. Oleic acid had an EC50 of 18.9 μg in this experimental setup (see Inset figure and Methods section). (d) Validation of the presence of phospholipids in cocoon shells. The same as above set of 10 contemporary (Black bars) and 10 old (White bars) cocoon shell samples were analysed for the presence of phospholipids as identified in the lipidomics analysis (see Fig. 5a). Results are expressed as μg of Phosphorus equivalents per cocoon shell. Potassium dihydrogen phosphate had an EC50 of 34.2 μg in this experimental setup (see Inset figure and Methods section).
