Figure 2
From: The role of Hfq in regulation of lipA expression in Pseudomonas protegens Pf-5
Effects of hfq overexpression on lipA expression. (A) Relative activity of whole-cell lipase in P. protegens Pf-5 wild-type and the hfq mutant. Whole-cell lipase activity was measured after bacterial culture in 50 mL LB growth reached the stationary phase. (B) Influence of hfq overexpression on the expression of the chromosome-borne lipA′-′lacZ construct in different strains. Bacteria were cultured in 50 mL LB to the stationary phase, and β-galactosidase activity was determined. Pf-5F3/pBBRKm: P. protegens Pf-5 wild-type with pBBRKm; Pf-5F3/pBBRK-hfq: P. protegens Pf-5 wild-type overexpressing hfq; PfΔhfqF3/pBBRK: hfq mutant with pBBRKm; and PfΔhfqF3/pBBRK-hfq: PfΔhfq complementary hfq mutant. Experiments were completed in triplicate. *P < 0.05, **P < 0.01 compared with the control group.
