Figure 6
From: The role of Hfq in regulation of lipA expression in Pseudomonas protegens Pf-5
Effect of rsmY on hfq regulation of lipA expression. (A) Verification of rsmY knockout by PCR. Lane 1: DNA markers. Lane 2: wild type strain. Lane 3: mutant strains (PfΔrsmY). (B) Relative activity of whole-cell lipase activity following hfq and rsmY single or double knockout. Whole-cell lipase activity was measured following bacterial culture to the stationary phase. The relative lipase activity decreased to a greater extent following double knockout, than that observed in the single-gene knockout strain, indicating that hfq and rsmY both regulate lipase expression and activity, and that RsmY is capable of partially compensating for the effects of hfq knockout. (C) β-galactosidase activity following hfq and rsmY single or double knockout. β-galactosidase activity was determined following bacterial culture to the stationary phase. β-galactosidase activity was much lower in the double-knockout strain, and overexpression of rsmY in the hfq mutant enhanced β-galactosidase activity, indicating that the effects of hfq knockout on lipA expression was compensated for by rsmY expression. Experiments were completed in triplicate. *P < 0.05, **P < 0.01 compared with the control group.
