Figure 4

D-JNKi reduces JNK activation in neuronal p38α knockout mice to levels in control mice. (A) 293 T cells were treated with D-JNKi (1 μM), SP600125 (10 μM) for 30 minutes at 37 °C. After stimulation with anisomycin (ANI; 25 ng/ml for 30 minutes) or vehicle (VEH), cell lysates were prepared for immunoblots for pThr183/pTyr185 JNK (pJNK), JNK. GAPDH, loading control. (B) Quantification of immunoblots in (A). (n = 3) (Student’s t-test) ^***p < 0.001. (C) Immunoblots of hippocampal lysates from p38α^lox/lox and p38α^ΔNeu mice 30 minutes post-application (i.p.) of D-JNKi (0.3 mg/kg body weight) or control vehicle (VEH). Immunoblots were probed for pThr183/pTyr185 JNK (pJNK), JNK, p38α and Gapdh. 30 minutes post injection of D-JNKi, activation levels of JNK were comparable between p38α^lox/lox and p38α^ΔNeu brains, whereas control-treated p38α^ΔNeu brains show consistently higher levels of active JNK than control-treated p38α^lox/lox. (n = 5) (D) Quantification of immunoblots in (A). (n = 5) (Student’s t-test) ^***p < 0.001.