Figure 1 | Scientific Reports

Figure 1

From: Covalent coupling of Spike’s receptor binding domain to a multimeric carrier produces a high immune response against SARS-CoV-2

Figure 1

The strategy: Sortase A-mediated transpeptidation reaction. (A) The reaction is exemplified by the coupling of a BLS subunit (red) and the SARS-Co-V2 RBD domain (green). The enzymatic intermediary form (RBD covalent bound to Sortase A) is shown, as well as its subsequent hydrolysis to yield the by-product RBD-Leu-Pro-Glu-Thr. The latter cannot be reused in the coupling reaction. The reaction catalyzed by TEV protease to yield Gly-Gly-Gly-BLS, the substrate of Sortase A is also shown. (B) A hypothetical reaction product with a multiplicity of 10:10 (RBD10/BLS10) is shown.

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