Figure 6 | Scientific Reports

Figure 6

From: Covalent coupling of Spike’s receptor binding domain to a multimeric carrier produces a high immune response against SARS-CoV-2

Figure 6

RBD-specific humoral immune response in mice vaccinated with monomeric RBD and RBD-BLS multimers. (A) Immunization scheme and blood collection. BALB/c mice (n = 5) were vaccinated with two doses of the formulations 30 days apart subcutaneously, and sera were obtained 20 days after the boost. Created with BioRender.com. (B) Evaluation of anti-RBD IgG antibody titers. Serial dilutions of sera samples were dispensed in a multi-well plate and RBD-specific IgG titers were determined by ELISA at day 50. The addition or not of 500 μg of Al(OH)3 adjuvant is indicated. The results are expressed as mean endpoint titers. The RBD + BLS group wasco-immunized with BLS and RBD at the same concentration as used in the RBD-BLS HM group, but not coupled. Control and PreIS correspond to normal mouse and preimmune sera, respectively. The S.E.M. is indicated by vertical lines. Differences were considered statistically significant when p < 0.05 (*, Student’s t test). Black dots show the individual values corresponding to the RBD-specific IgG titer.

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