Figure 7 | Scientific Reports

Figure 7

From: Covalent coupling of Spike’s receptor binding domain to a multimeric carrier produces a high immune response against SARS-CoV-2

Figure 7

SARS-CoV-2 S pseudotyped virus neutralization assay. (A) Scheme of the assay. HEK-293T cells transfected with ACE2 and TMPRSS2 protease were transduced with a SARS-CoV-2 S pseudotyped lentivirus carrying a GFP-encoding mRNA in the presence of different dilutions of mouse sera. Forty eight hours later, cells were observed under the microscope. Created with BioRender.com. (B) The number of GFP positive cells for each serial dilution was determined. The serum antibody dilution causing a 50% reduction of GFP positive cells (IC50) compared to control “virus only” treated cells was calculated using Graphpad Prism. PreIS corresponds to the preimmune sera.

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