Figure 2
From: DCZ19931, a novel multi-targeting kinase inhibitor, inhibits ocular neovascularization
DCZ19931 has no obvious cytotoxicity and tissue toxicity. (A–C) HUVECs were incubated with the test concentrations of DCZ19931 (1 nM to 10 μM) or left untreated (Ctrl) for 24 h. Cell viability was measured by MTT assays (A, n = 4). Flow cytometry using Annexin V-FITC/PI double staining (B, n = 4) and Calcein-AM/PI staining (C, n = 4, scale bar, 20 μm) was performed to detect the apoptosis of HUVECs. (D,E) C57BL/6J mice received intravitreal injections of PBS (2 μL, Ctrl), 10% DMSO (2 μL), or DCZ19931 (2 μL, 1 μg/μL) for seven days. H&E staining (D, n = 5 animals per group, scale bar, 50 μm) and TUNEL staining assays were performed to detect retinal histological changes and retinal cell apoptosis (E, n = 5 animals per group, scale bar, 50 μm). DNase I was detected as a positive control in TUNEL staining assays. Statistical significance was evaluated by one-way ANOVA followed by Bonferroni post hoc test. *P < 0.05 indicated significant difference between the marked groups.
