Figure 2

Shrimp immune gene expression of peroxinectin (PX), serine protease (SP), prophenoloxidase-1 (proPO I), and anti-lipopolysaccharide factor-1 (ALF-1). Shrimp’s regimen with 4.5 × 1010 [100X] CFU/2 L seawater of L. johnsonii KD1 for five days with n = 30 shrimps per replicate (two replicates/group) while the negative group without probiotic treatments with n = 30 shrimps per replicate (two replicates/group). Mean of fold difference of ± standard error with n = 4 shrimps per group at day 6 (or one day post-probiotics regimen in treated groups) and day 8 (or three days post-probiotics regimen). Fold difference of 1 represented as dotted line indicates no change in shrimp gene expression in probiotic treatment in comparison to without probiotic treatment (negative).