Table 5 Ranking of the reference gene candidates generated according to their stability values calculated by the geNorm, NormFinder, BestKeeper, Delta-Ct, and RefFinder algorithms using the Cq (Cycle of Quantification) data obtained from stem, leaves, and leaf apex of eucalyptus inoculated with B. bassiana and plants infested with L. invasa.
Gene | geNorm | NormFinder | BestKeeper | Delta-Ct | RefFinder | |||||
|---|---|---|---|---|---|---|---|---|---|---|
M stability value | Ranking | SV stability value | Ranking | SD stability value | M stability value | Ranking | SV stability value | Ranking | SD stability value | |
EcPP2A-1 | 0.264 | 1 | 0.151 | 2 | 1.245 | 5 | 0.477 | 2 | 2.115 | 1 |
EcUPL7 | 0.291 | 3 | 0.124 | 1 | 1.262 | 6 | 0.476 | 1 | 2.213 | 2 |
EcSAND | 0.264 | 1 | 0.212 | 4 | 1.091 | 3 | 0.490 | 3 | 2.449 | 3 |
EcEUC12 | 0.274 | 2 | 0.210 | 3 | 1.220 | 4 | 0.493 | 4 | 3.464 | 4 |
EcIDH | 0.349 | 4 | 0.438 | 7 | 0.994 | 2 | 0.604 | 7 | 4.705 | 5 |
EcUBP6 | 0.609 | 10 | 0.870 | 11 | 0.782 | 1 | 0.931 | 11 | 6.040 | 6 |
Ecα-TUB | 0.424 | 6 | 0.395 | 5 | 1.503 | 8 | 0.589 | 5 | 6.117 | 7 |
EcPTB | 0.387 | 5 | 0.405 | 6 | 1.274 | 7 | 0.590 | 6 | 6.236 | 8 |
EcACT | 0.451 | 7 | 0.447 | 8 | 1.561 | 9 | 0.612 | 8 | 8.239 | 9 |
EcEF1-α | 0.499 | 8 | 0.561 | 9 | 1.571 | 10 | 0.683 | 9 | 9.240 | 10 |
EcH2B | 0.537 | 9 | 0.666 | 10 | 1.605 | 11 | 0.753 | 10 | 10.241 | 11 |
