Table 6 RT-qPCR primer sequences, gene name, accession number, melting temperature (Tm), amplicon size, correlation coefficient (R2), and amplification efficiency (E%) of the reference and target genes analyzed.
Gene name | Accession number | Primer sequence (5′–3′) | Tm (°C) | Amplicon (bp) | R2 | E (%) |
|---|---|---|---|---|---|---|
EcPP2A-1 | EcC035070.20 | Fw: GGAGATTGGAGAGAACATGGACC Rv: GGAGTCTTGCGTGTGGTGTC | 62.5 60.7 | 87 | 0.99 | 100.0 |
EcEF-1α | EcC054749.90 | Fw: TCGGGCTTTGAGGGTGACAA Rv: TCTTGGGCTCTAGGATCAGGTC | 61.6 62.8 | 103 | 1.00 | 97.7 |
EcPTB | EcC038236.10 | Fw: TTTCTGCTTTTGGTCCTGTGC Rv: GCAGCAGTCGTTATGTCAGGG | 60.8 62.5 | 94 | 0.99 | 96.8 |
EcACT | EcS556981.10 | Fw: GCTGGATTCGCAGGTGATGATG Rv: CCTTCTGACCCATTCCGACCA | 62.8 64.8 | 97 | 1.00 | 100.0 |
EcIDH | EcC000103.10 | Fw: AGCGAGGGAGGTTATGTGTGG Rv: ATGTCATCAAGCCAAGCGATCC | 63.7 61.9 | 97 | 0.98 | 96.2 |
Ecα-TUB | EcC009196.10 | Fw: GGGGATTCAGGTCGGCAACT Rv: TGAGGTGTCACTGGGCATCG | 62.3 61.7 | 88 | 1.00 | 95.1 |
EcUBC2 | EcS510545.10 | Fw: TGCTCTTATCAAGGGACCGTCG Rv: GAAACGAACTTGAGGCGGCT | 63.7 60.1 | 106 | 0.99 | 100.0 |
EcEUC12 | EcC054911.20 | Fw: TACCCTGTGAGAGTGCTGCC Rv: GCACATCTCCCGCTCATCCT | 60.7 61.2 | 90 | 0.99 | 97.4 |
EcH2B | EcC049406.40 | Fw: CCTGCTCGATTCCTGATGGC Rv: CACCGCCGACTTCTTCTCCT | 60.3 61.6 | 81 | 0.99 | 100.0 |
EcSAND | EcC020159.20 | Fw: TGAGGAAGTGAGGAGTGACGC Rv: CATCATCCTCATCAACGTGCCG | 63.8 63.1 | 80 | 0.99 | 99.3 |
EcUPL7 | EcC023921.10 | Fw: ACTGAACCCTGTGGCTGGAT Rv: GCGATCAGAGGCACCATGAAA | 60.4 62.2 | 118 | 0.97 | 83.1 |
EcDREB2 | EcC047161.20 | Fw: GAGGTGACGGAGGTGAAGGG Rv: GCAACGAAACGCACTCCCAC | 63.0 61.7 | 118 | 1.00 | 87.4 |
