Figure 4
From: Effective combination of cold physical plasma and chemotherapy against Ewing sarcoma cells in vitro

Apoptosis induction by the combination of CPP and cytostatic agents. To evaluate the apoptotic effect of combination therapy of the cell lines A673 and RD-ES Caspase 3/7 activity assay and TUNEL assay were used. The relative Caspase 3/7 signals and TUNEL signals of cells treated with CPP and combination therapy were normalized to the relative Caspase 3/7 signals and TUNEL signals of cells treated with Argon (control). The mean values ± SD are depicted in the graphs and were assessed for statistically significant differences using paired t-tests (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001).