Figure 5
From: Effective combination of cold physical plasma and chemotherapy against Ewing sarcoma cells in vitro
DNA damage marker formation upon CPP and chemotherapeutics treatment. A673 cells were stained 24 h after treatment with CPP, CIS, MTX, or combinations of these (A) to investigate the formation of nuclear pATM and yH2AX foci (B). Images were quantified by calculating the mean fluorescence intensity (MFI) of pATM (C) and yH2AX (D) in all detected nuclei of treated cells against untreated controls. The mean values ± SD are depicted in the graphs and were assessed for statistically significant differences using one-way ANOVA and Šídák's multiple comparisons test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001).
