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Figure 1

From: Essential oils from Colombian Croton spp. exhibit antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and ampicillin- and streptomycin-resistant Escherichia coli

Figure 1

Representation of results for determination of MIC and MBC of C. hondensis essential oils against the four bacterial strains under study. (a) Representation of results for determination of MIC from essential oil of C. hondensis against the four studied bacterial strains. (a1) Map of MIC, (a2) sensitive S. aureus, (a3) Resistant S. aureus, (a4) Sensitive E. coli, (a5) Resistant E. coli, (a6) Controls: C + (inhibition control): culture medium + bacterial inoculum + 10 µL of Lincomycin 630 mg/2 mL or 10 µL of gentamicin 160 mg/2 mL for S. aureus and E. coli strains, respectively; C− (growth control): culture medium + bacterial inoculum. (b) Representation of results for MBC determination from essential oil of C. hondensis against S. aureus ATCC 29213. (b1–b4) Determination of bacterial inhibition or MBC by different essential oil concentrations. (b5) Inoculum control, to validate the final bacterial density inoculated for well in the MIC determinations. (b6) Growth control to validate the viability of bacteria in essential oil absence. The image for the growth control was obtained under a different background setting compared to the remaining figures. This variation does not impact the interpretation of the experimental results. All tests were conducted in triplicate.

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