Figure 1 | Scientific Reports

Figure 1

From: Reverse genetic approaches allowing the characterization of the rabies virus street strain belonging to the SEA4 subclade

Figure 1

Growth efficiency of the recombinant RABV Toyohashi strain (rToyo) in cell cultures. (A) Schematic representation of the plasmid construct encoding the wild-type Toyohashi strain (Toyohashi, GenBank LC812291, 11,922 nt in size). The full genome cDNA is flanked by the T7 promoter sequence and the antigenomic hepatitis delta virus ribozyme (HDV-Rbz) in a pUC19 vector. (B) Mouse neuroblastoma (NA) cells and mouse myoblasts (G-8) were inoculated with Toyohashi (blue square) or rToyo (red circle) at a multiplicity of infection (MOI) of 0.01. The culture supernatants containing progeny viruses were collected at the indicated time points and titrated using a focus forming assay. Means ± standard deviations of triplicate data are shown in the graph. Statistics were calculated by a multiple t-test with Welch correction. *p < 0.05; ns, not significant (p ≧ 0.05).

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