Figure 3 | Scientific Reports

Figure 3

From: AUTS2 disruption causes neuronal differentiation defects in human cerebral organoids through hyperactivation of the WNT/β-catenin pathway

Figure 3

AUTS2 regulates cell fate commitment by promoting neuron differentiation and repressing ChP generation. (A) tSNE plot of 1111 cells from AUTS2+/- and AUTS2-/- 75-day-old cerebral organoids, colored by the annotated cell types (C0-C8). (B) tSNE plot of AUTS2+/- and AUTS2-/- 75-day-old cerebral organoids, colored by the genotypes. For each genotype, three biologically replicated samples (n = 3) were processed for scRNA-seq, each including 3 cerebral organoids. (C) Heatmap of selected marker genes for neurons, forebrain, proliferation, retina, neuroepithelial cells, and ChP epithelial cells. Clusters and genotypes are color-labeled on top of the heatmap. (D) Features plot of AUTS2, forebrain marker (FOXG1), retinal neurons (RCVRN), and ChP epithelial (TTR). (E) The violin plot indicates the upregulation of ChP markers (TTR, CLIC6, AQP1, HTR2C and ICFBP7) in AUTS2-/- cerebral organoids as compared to AUTS2+/- cerebral organoids. (F) The violin plot indicates the upregulation of ChP markers (TTR, CLIC6, AQP1, HTR2C and ICFBP7) in C0 and C6.

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