Fig. 3 | Scientific Reports

Fig. 3

From: Blocking antibodies against integrin-α3, -αM, and -αMβ2 de-differentiate myofibroblasts, and improve lung fibrosis and kidney fibrosis

Fig. 3

Conjugation of decorin’s collagen-binding peptide (CBP) to anti-integrin-αM (α-αM) increases antibody concentration in fibrotic lungs. Mice were intranasally instilled with 75 μg of bleomycin sulfate (fibrotic) or PBS (healthy) and injected 1 week later with Cy7-α-αM or Cy7-CBP-α-αM. (A) Heart, lung, spleen, kidneys, and liver were harvested 48 h after injection, and fluorescence intensity was measured via IVIS. (B) The number of photons from lungs or (C) spleen. Fluorescence of the resected organs was pooled and the percentage of fluorescence associated with (D) lungs and (E) spleen. n ranges from 2 to 4. * = statistical significance of P < 0.05, < 0.01, or < 0.001, significance is fluorescence of fibrotic lungs vs fluorescence of healthy lungs, Student’s t-test.

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