Fig. 1
From: LMK-235 suppresses osteoclastogenesis and promotes osteoblastogenesis by inhibiting HDAC4
LMK-235 inhibits RANKL-induced osteoclast generation. (A) Five days after RANKL stimulation, the expression of HDAC4, HDAC5 and osteoclast-specific genes ACP-5, DC-STAMP and TNFRSF11a were assessed by qRT-PCR, and GAPDH served as the reference gene (n = 3). (B) CCK-8 assay was employed to evaluate the cytotoxicity of LMK-235 on BMMs (n = 3). (C) After six days of RANKL stimulation, TRAP staining results of OCs. (D) Quantitative analysis of TRAP-positive OCs with three or more nuclei (n = 3). (E) The molecular structural formula of LMK-235. *P < 0.05, **P < 0.01, ***P < 0.001 represents comparison with the control group.
