Table 2 Experimental matrix for the 25−1 FFD and responses for iron uptake and growth.

From: Medium optimization to improve growth and iron uptake by Bacillus tequilensis ASFS1 using fractional factorial designs

Run

Coded levels

Response 1

Response 2

Fe content in biomass (μg mL−1)

Dried biomass (mg mL−1)

X1

X2

X3

X4

X5

Experimental

Predicted

Experimental

Predicted

1

− 1

− 1

− 1

− 1

 + 1

96.9

89.5

1.2

1.5

2

 + 1

− 1

− 1

− 1

− 1

71.5

69.3

2.3

2.05

3

− 1

 + 1

− 1

− 1

-1

60.7

49.7

1.4

1.65

4

 + 1

 + 1

− 1

− 1

 + 1

65.5

86.6

3.3

2.5

5

− 1

− 1

 + 1

− 1

− 1

44.3

34.08

1

0.98

6

 + 1

− 1

 + 1

− 1

 + 1

122.9

114.3

2.4

2.6

7

− 1

 + 1

 + 1

− 1

 + 1

79

94.7

2

2.2

8

 + 1

 + 1

 + 1

− 1

− 1

27.9

31.3

1.5

1.97

9

− 1

− 1

− 1

 + 1

− 1

39.9

37.6

1

0.97

10

 + 1

− − 1

− 1

 + 1

 + 1

124.9

117.8

2.3

2.6

11

− 1

 + 1

− 1

 + 1

 + 1

97.8

98.2

2.3

2.2

12

 + 1

 + 1

− 1

 + 1

− 1

25.6

34.8

1.8

1.97

13

− 1

− 1

 + 1

 + 1

 + 1

110

130.1

1.8

1.52

14

 + 1

− 1

 + 1

 + 1

− 1

91.8

109.96

2.3

2.05

15

− 1

 + 1

 + 1

 + 1

− 1

95

90.4

2

1.65

16

 + 1

 + 1

 + 1

 + 1

 + 1

160.8

127.3

2.4

2.52

17

0

0

0

0

0

96.2

82.23

2.2

1.94

18

0

0

0

0

0

95.8

82.23

2.3

1.94

19

0

0

0

0

0

95.7

82.23

2.2

1.94