Table 2 Experimental matrix for the 25−1 FFD and responses for iron uptake and growth.
Run | Coded levels | Response 1 | Response 2 | ||||||
|---|---|---|---|---|---|---|---|---|---|
Fe content in biomass (μg mL−1) | Dried biomass (mg mL−1) | ||||||||
X1 | X2 | X3 | X4 | X5 | Experimental | Predicted | Experimental | Predicted | |
1 | − 1 | − 1 | − 1 | − 1 | + 1 | 96.9 | 89.5 | 1.2 | 1.5 |
2 | + 1 | − 1 | − 1 | − 1 | − 1 | 71.5 | 69.3 | 2.3 | 2.05 |
3 | − 1 | + 1 | − 1 | − 1 | -1 | 60.7 | 49.7 | 1.4 | 1.65 |
4 | + 1 | + 1 | − 1 | − 1 | + 1 | 65.5 | 86.6 | 3.3 | 2.5 |
5 | − 1 | − 1 | + 1 | − 1 | − 1 | 44.3 | 34.08 | 1 | 0.98 |
6 | + 1 | − 1 | + 1 | − 1 | + 1 | 122.9 | 114.3 | 2.4 | 2.6 |
7 | − 1 | + 1 | + 1 | − 1 | + 1 | 79 | 94.7 | 2 | 2.2 |
8 | + 1 | + 1 | + 1 | − 1 | − 1 | 27.9 | 31.3 | 1.5 | 1.97 |
9 | − 1 | − 1 | − 1 | + 1 | − 1 | 39.9 | 37.6 | 1 | 0.97 |
10 | + 1 | − − 1 | − 1 | + 1 | + 1 | 124.9 | 117.8 | 2.3 | 2.6 |
11 | − 1 | + 1 | − 1 | + 1 | + 1 | 97.8 | 98.2 | 2.3 | 2.2 |
12 | + 1 | + 1 | − 1 | + 1 | − 1 | 25.6 | 34.8 | 1.8 | 1.97 |
13 | − 1 | − 1 | + 1 | + 1 | + 1 | 110 | 130.1 | 1.8 | 1.52 |
14 | + 1 | − 1 | + 1 | + 1 | − 1 | 91.8 | 109.96 | 2.3 | 2.05 |
15 | − 1 | + 1 | + 1 | + 1 | − 1 | 95 | 90.4 | 2 | 1.65 |
16 | + 1 | + 1 | + 1 | + 1 | + 1 | 160.8 | 127.3 | 2.4 | 2.52 |
17 | 0 | 0 | 0 | 0 | 0 | 96.2 | 82.23 | 2.2 | 1.94 |
18 | 0 | 0 | 0 | 0 | 0 | 95.8 | 82.23 | 2.3 | 1.94 |
19 | 0 | 0 | 0 | 0 | 0 | 95.7 | 82.23 | 2.2 | 1.94 |