Fig. 1 | Scientific Reports

Fig. 1

From: Circular RNA HIPK3 mediates epithelial–mesenchymal transition of retinal pigment epithelial cells by sponging multiple microRNAs

Fig. 1

Expression of circHIPK3 is increased in RPE-EMT model in vitro. (A) Bright field images of RPE cells treated or untreated with TGF-β1. Scale bar, 100 μm. (B) Expression of EMT-related markers detected by qRT-PCR (normalized to GAPDH expression, n = 3). *P < 0.05 versus Ctrl group. (C) Expression of EMT-related markers detected by immunofluorescence staining (n = 3). Scale bar, 50 μm. (D) Transwell migration assays in RPE cells treated or untreated with TGF-β1 (n = 5). Scale bar, 50 μm. (E) Analysis of migrated cell number in the Transwell migration assays (n = 5). *P < 0.05 versus Ctrl group. (F) Expression pattern of circHIPK3 in RPE cells detected by RNA-FISH assay. Scale bar, 50 μm. (G) Expression of circHIPK3 treated with TGF-β1 for 0 h, 12 h, 24 h, and 48 h detected by qRT-PCR (normalized to 18S rRNA expression, n = 3). *P < 0.05 versus Ctrl group.

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