Fig. 5 | Scientific Reports

Fig. 5

From: Circular RNA HIPK3 mediates epithelial–mesenchymal transition of retinal pigment epithelial cells by sponging multiple microRNAs

Fig. 5

circHIPK3 regulates RPE-EMT process partly through circHIPK3/miR-106b-5p axis. (A) qRT-PCR analysis of the expression of different miRNAs in RPE cells treated with si-circHIPK3 or lv-circHIPK3 (normalized to U6 snRNA expression, n = 3). *P < 0.05 versus Ctrl group. (B) qRT-PCR analysis of the expression of miR‐106a-5p and miR‐106b-5p in normal RPE cells (normalized to U6 snRNA expression, n = 3). *P < 0.05 versus miR‐106a-5p group. (C) qRT-PCR analysis of the expression of circHIPK3 in RPE cell lysates after biotin‐coupled miR‐106a-5p and miR‐106b-5p pull‐down assays (n = 3). *P < 0.05 versus miR‐106a-5p group. (D) Expression of EMT-related markers detected by qRT-PCR (normalized to GAPDH expression, n = 3). *P < 0.05. (E) Transwell migration assays in RPE cells treated with Scr mimic, miR-106b mimic, miR-106b mimic + lv-Scr, or miR-106b mimic + lv-circHIPK3 (n = 5). Scale bar, 50 μm. (F) Analysis of migrated cell number in the Transwell migration assays (n = 5). *P < 0.05 versus Ctrl group.

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