Fig. 6

Inhibition of CIAPIN1 enhances breast cancer cell apoptosis. MDA-MB-231 and MDA-MB-453 cells were transfected with siRNA targeting CIAPIN1 mRNA (siRNA-299) for a further 24 h culture. (A) Cell apoptosis was assessed by Annexin V-FITC double staining and analyzed by flow cytometry. The representative images of flow cytometry are shown. (B) The apoptotic rate was calculated by the sum of the early apoptotic cells (lower right quadrant ) and the late apoptotic cells (upper right quadrant). Data represent the average of three independent experiments (mean ± SD), and analyzed using Student t test.. ***P < 0.001 vs. si-NC group.