Fig. 9 | Scientific Reports

Fig. 9

From: Downregulation of CIAPIN1 regulates the proliferation, migration and glycolysis of breast cancer cells via inhibition of STAT3 pathway

Fig. 9

Overexpression of CIAPIN1 promotes glycolysis in breast cancer cells through STAT3/PKM2 pathway. The MCF7 and MDA-MB-468 cells were overexpressed with CIAPIN1 and co-incubated with a STAT3 inhibitor STAT3-IN-3 (1 μM; HY-128588, MedChemExpress) for further 24 h. (A) Representative gel blots depicting levels of PKM2 (normalized to GAPDH) and phosphorylated STAT3 (p-STAT3, normalized to total STAT3). (B, C) Quantification analysis shows that inhibition of CIAPIN1 reduced the PKM2 protein expression and phosphorylation of STAT3. The energy metabolism by overexpression of CIAPIN1 was measured in the lysate of MCF7 and MDA-MB-468 cells on (D) pyruvate, (E) lactate and (F) ATP. Data represent the average of three independent experiments (mean ± SD). ***P < 0.001 vs. Vector group; ###P < 0.001 vs. CIAPIN1-OE group.

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