Fig. 8 | Scientific Reports

Fig. 8

From: Quercetin inhibited LPS-induced cytokine storm by interacting with the AKT1-FoxO1 and Keap1-Nrf2 signaling pathway in macrophages

Fig. 8

Effects of quercetin on intracellular ROS levels under LPS induction at 24 and 48 h. (A, B) Flow cytometry results showed that quercetin enhanced the scavenging of intracellular ROS with increasing concentration and time under LPS induction. (C, D) The intracellular ROS content was observed by fluorescence microscopy, and green fluorescence represents ROS. Magnification is 20×. (E) Western blotting analysis revealed that Keap1 protein expression was down-regulated and Nrf2 protein expression was up-regulated in Raw264.7 cells treated with LPS and quercetin. (F, G) The expression levels of Keap1 and Nrf2 were normalized to their respective GAPDH at 24 and 48 h. Original blots are presented in Supplementary Fig. 2E, F. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared with the LPS group.

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