Fig. 1

Forward genetic screen identifies ATPAT1 as target of periplogenin. (A) Schematic of the forward genetic screen approach. (B) Forward genetic screen results. The -log₁₀(p-value) of the analysis in the screen was plotted on the y-axis and 1572 candidate genes were organized by chromosome position on x-axis, the size of the dot exhibits the clone numbers containing the identified mutations of corresponding gene. (C, D) Molecular docking of ATP1A1 with periplogenin. (E) The effect of periplogenin treatment on the Na+ /K +-ATPase activity. The cell lysates were derived from wild-type DU145 cells (WT) and CRISPR/Cas9 engineered DU145 cell clones with T804A substitution (T804A). * and ** indicate p values of < 0.05, < 0.01, respectively.