Fig. 4
IL-1-stimulated NF-κB activity in Low and High Synovitis cell superlots. (a) NF-κB activity assay—schematic showing viral construct driving IL-1 mediated luciferase (Luc) expression under control of NF-κB response elements. RE: regulatory element; NRE: negative regulatory element (b) NF-κB activity due to IL-1 dosing in low and high synovitis superlot cells expressed as fold-change of luminescence units relative to control at 4 h (left), 8 h (middle), and 12 h (right). (c) IL-1RA modulation of IL-1-mediated NF-κB response in low and high synovitis superlot cells expressed in fold-change of luminescence units relative to control at 8 h with IL-1RA. Data presented as mean + SEM (n = 6 per group) with statistical analysis performed using two-way ANOVA with Tukey's post-hoc, *p = 0.0187, **p ≤ 0.0079, ***p ≤ 0.006, ****p < 0.0001. 
