Fig. 6
Chondrocyte gene expression and Ingenuity Pathway Analysis differentially regulated by osteochondral injury mode and synovial cell co-culture. Relative gene expression for (a) Injury mode (no injury, compression, fracture) and co-culture with synovial cells (No Cells, Low synovitis, High synovitis) compared to Low synovitis cells and No injury, representing “normal joint” control group and (b) direct comparisons of injury modes, compression (C) and fracture (Fx), co-cultured with Low or High synovitis cells. Gene expression data (n = 3 per group) presented as fold change (FC) with statistical analysis performed using two-way ANOVA with Bonferroni’s correction for multiple comparisons (adjusted p-values reported). Genes highlighted in orange are significantly upregulated, and genes highlighted in blue are significantly downregulated. Significant p-values shown in red. Ingenuity pathway analysis activation z-scores with B–H corrected p-values for top canonical pathways enriched by (c) injury mode (no injury, compression, fracture) and co-culture with synovial cells (no cells, low synovitis, high synovitis) compared to low synovitis cells and no injury, representing “normal joint” control group; and (d) Direct comparisons of compression (C) and fracture (Fx) injury modes co-cultured with low or high synovitis cells.
