Fig. 4

Changes in the expression of autophagy-related proteins and the lncRNA NBR2 and the number of autophagosomes in breast cancer cells treated with the lncRNA NBR2 under starvation conditions. (A) The relative levels of autophagy related proteins such as LC3 and P62 in BT-549 and MDA-MB-231 cells after 0 h, 6 h, 9 h and 12 h were detected by Western blotting. (B) Changes in lncRNA NBR2 expression were verified by qRT-PCR in BT-549 and MDA-MB-231 cells after 6 h of starvation. (C) Changes in the number of autophagosomes in BT-549 and MDA-MB-231 cells were detected by immunofluorescence experiments after 6 h of starvation. (D,E) Western blotting analysis was used to determine the changes in autophagy-related proteins such as LC3 and P62 in BT-549 and MDA-MB-231 cells after 6 h of starvation and downregulation/upregulation of the lncRNA NBR2. (F,G) Immunofluorescence assays were used to detect the number of autophagosomes in BT-549 and MDA-MB-231 cells after lncRNA NBR2 upregulation or downregulation after 6 h of starvation. *P < 0.05, **P < 0.01, ***P < 0.001.